The vector supplied is predigested with ecor i and xho i restriction enzymes and is ciaptreated to reduce the background of. New 1433associated proteins involved in cytoskeletal rearrangements and. Introduction the pbluescript ii phagemids plasmids with a phage origin are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for dna sequencing, generation of rna transcripts in vitro and sitespecific mutagenesis and gene mapping. Problems with transformation pbluescript plasmid dna i have a gene, apoe, and transformed it into pbluescript digested with ecorv with suitable antibiotic in the right concentration. Caenorhabditis elegans sex determination gene mog1. Caenorhabditis elegans rab3 mutant synapses exhibit impaired. We suggest using a current version of chrome, firefox, or safari.
Every day thousands of users submit information to us about which programs they use to open specific types of files. Caenorhabditis elegans sex determination gene mog1 encodes a. This online pdf compressor allows to compress pdf files without degrading the resolution dpi, thus keeping your files printable and zoomable. Cloning, sequencing and complementation analysis of the. Thiscorrespondedexactlyto exon6 ofthe urod gene fig. The role of a purinespecific nucleoside hydrolase in. A 450 bp fragment sacwdray was derived from the 5upstream region of the ggapdh i gene fig. The role of a purinespecific nucleoside hydrolase in spore germination of bacillus thuringiensis liang liang,1,2 xihong he,1,2 gang liu1 and huarong tan1 correspondence. Download a pdf containing pricing for our full product list. Materials and methods strains, media, and growth conditions fungal strains used in this study are listed in supporting table 1. Ecori cbe28 and 51 or bamhi cbe76cut pbluescript ks stratagene after.
The ribosomal protein l32 riboprobe, used as an internal standard, was a generous gift of dr. Component substitution may result in lowerefficiency library construction. By continuing to use this site, you agree to the use of cookies. Cyclosporin aresistance based gene placement system for. Transgenic mouse proteomics identifies new 1433associated proteins involved in cytoskeletal rearrangements and cell signaling s pierreolivier angrand, inmaculada segura, pamela vo. Genetic regulation and cellular expression1 gian b. Cell identification was based on the characteristic morphology and position.
An alternative pathway to bcarotene formation in plant chromoplasts discovered by mapbased cloning of betaand oldgoldcolor mutations in tomato gil ronen, lea carmelgoren, dani zamir, and joseph hirschberg department of genetics, the life sciences institute, and faculty of agriculture, the hebrew university of jerusalem, jerusalem, 91904 israel. Total rna preparations were extracted from50 mg of b6d2f2 mouse embryonic tissues with rnazol b leedo medical laboratories, houston, tx following the manufacturers protocol. Phagemids represent a hybrid type of class of vectors that serve to produce singlestranded dna. Isolation and characterization of a mouse homolog of the. Have questions about your order, deposit, or a plasmid. Subsequently a tap cassette from pzome1 and the gateway recombination cassette invitrogen were inserted between the promoter and. Transgenic mouse proteomics identifies new 1433associated. Mutagenic primers were used to generate all point mutations and deletions with the geneeditor kit promega according to the manufacturer recommendations and as previously described 17. Isolation and characterization of mammalian homologs of.
Dnaform precision gene technologies leading venture plaza2 751, onocho, tsurumiku, yokohama city. See the list of programs recommended by our users below. Receive the latest news, hot plasmids, discounts and more. This was subcloned into the sacwxbal bluntended site of pt 1 to produce. Problems with transformation pbluescript plasmid dna. The polylinker sequence is located within a lacz controlled gene designed to.
The bamhi fragments were ligated and introduced into escherichia coli, and transformants containing pbluescript ks 1 sequences were selected on ampicillincontaining medium. The sequences include a polylinker sequence mcs, antibiotic resistance sequence to ampicillin and an e. The sequences include a multiple cloning site sequence mcs, antibiotic resistance sequence to ampicillin and an e. Human cardiac kcnj2 was obtained in the plasmid pbluescript ks as a gift from carol vandenberg. This is a free resource for the scientific community that is compiled by addgene. The template for the parathyroid hormone pth gene probe a kind gift from b. As cloning plasmid pbluescript ii ks stratagene and 2. The map, notes, and annotations on this page and in the sequencemap file are ed material. Learn about the latest plasmid technologies and research tools. Modified bean seed protein phaseolin did not accumulate. A bglll site was created at nsil site by digestion of ptsk1 with nsil and insertion of an nsi1bglll linker annealed oligonucleotides 5tagatcttgca3 and 5agatctatgca3 that has nsil compatible ends. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. We notice that you are using internet explorer, which is not supported by xenbase and may cause the site to display incorrectly.
Cyp2ba 450bp hind111 fragment of p7a22e 11 encoding a region shown to be devoid of the repetitive sequences present elsewhere in the cdna 1 1 was inserted into the hind111 site of pbluescript ks to form pbs2b450. Compress pdf files for publishing on web pages, sharing in social networks or sending by email. Rna extraction, reverse transcription rtpcr, and northern analysis. Sac l not l xba l spe l bamh l sma l pst l ecor l ecor v hind lll sal l xho l kpn l pbluescript ll ks 3. Phagemid cloning vector, also known as bluescribe sk minus or blueskm. Elsevier fems microbiology letters 144 1996 5359 cloning, sequencing and complementation analysis the reca gene from prevotella ruminicola rustem i. In genetics, pbluescript pbs or pbluescript ii is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. The csr825975f primer contains an artiwcial sacii site while the csr827023r primer is located. A shuttle vector which facilitates the expression of. The synthesized genes next were subcloned into the plantexpression binary vector, pbinjit ferrandiz et al. Cloning, sequencing and complementation analysis of the reca. From 101 plasmids, 11 cbes were obtained, and of these, 4 had sequences related to cbe28.
The ligation products were used to trans form escherichia coli through electroporation carried out by the biorad gene pulser biorad lab oratories, richmond, ca set at i. The quick and simple way to handle your files is to get a quality piece of software, such as reaconverter. The ampicillinresistanceencoding gene ap r of pbluescript has been replaced by genes encoding resistance to either kanamycin km r or tetracycline tc r. This software is extremely efficient in managing a wide range of batch conversions. Get a printable copy pdf file of the complete article 104k, or click on a page image below to browse page by page. This was subcloned into the sacwxbal bluntended site of pt 1 to produce construct ptex5. Materials provided materials provideda quantity storage. A new set of useful cloning and expression vectors derived. From 101 plasmids, 11 cbes were obtained, and of these, 4 had sequences related to. Caenorhabditis elegans rab3 mutant synapses exhibit.
While we do not yet have a description of the kcs file format and what it is normally used for, we do know which programs are known to open these files. Not a single difference was found between the sequence of the actual cdna clone and that predicted by the c. Sac3, an snf1like serinethreonine kinase that positively. Phagemids represent a hybrid type of class of vectors that serve to produc slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Convert pdx to pdf with reaconverter batch conversion software. The bluntend pcr products were cloned in plasmid pbluescript ii sk alting mees and short, 1989 digested with ecorv, and their dna sequence was.
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